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Porcine C3a (Complement Component 3a) ELISA Kit (AEES00704)

SKU:
AEES00704
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Porcine
Sensitivity:
0.47 ng/mL
Range:
0.78-50 ng/mL
$599
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Porcine C3a (Complement Component 3a) ELISA Kit
SKU:AEES00704
Target:Porcine C3a (Complement Component 3a)
Size:96T
Assay type:Sandwich-ELISA
Assay time:3.5h
Sensitivity:0.47 ng/mL
Detection range:0.78-50 ng/mL
Kit component:
ComponentQuantity (24 Assays)Quantity (96 Assays)Storage
Micro ELISA Plate (Dismountable)8 wells x 3 strips8 wells x 12 strips-20°C, 12 months
Reference Standard1 vial2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 60 µL1 vial, 120 µL
Concentrated HRP Conjugate (100×)1 vial, 60 µL1 vial, 120 µL-20°C (shading light), 12 months
Reference Standard & Sample Diluent1 vial, 20 mL1 vial, 20 mL4°C, 12 months
Biotinylated Detection Ab Diluent1 vial, 14 mL1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL1 vial, 30 mL
Substrate Reagent1 vial, 10 mL1 vial, 10 mL4°C (shading light)
Stop Solution1 vial, 10 mL1 vial, 10 mL4°C
Plate Sealer5 pieces5 pieces
Product Description1 copy1 copy

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine C3a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine C3a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine C3a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine C3a. You can calculate the concentration of Porcine C3a in the samples by comparing the OD of the samples to the standard curve.

Linearity:
Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)86-9991-10494-107
Average (%)9299100
1:4Range (%)87-9890-10094-106
Average (%)9395100
1:8Range (%)81-9295-10891-103
Average (%)8710097
1:16Range (%)82-9488-10193-107
Average (%)889598
Recovery:
Sample TypeRange (%)Average Recovery (%)
Serum (n=5)86-10193
EDTA plasma (n=5)95-108101
Cell culture media (n=5)97-107102
Precision:
Intra-assay PrecisionInter-assay Precision
Sample123123
n2020.020.020.020.020.0
Mean (ng/mL)2.525.0221.762.715.0719.82
Standard deviation0.140.251.030.180.240.65
C V (%)5.564.984.736.644.733.28
Sample type &Sample volume:; 100μL
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Porcine C3a concentrations in .
Specificity:This kit recognizes Porcine C3a in samples. No significant cross-reactivity or interference between Porcine C3a and analogues was observed.

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