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Porcine Bone Morphogenetic Protein 15 (BMP15) ELISA Kit

SKU:
PRDL00018
Product Type:
ELISA Kit
Sensitivity:
10.4pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
GDF9B,GDF9-B,ODG2,POF4,Growth/differentiation factor 9B
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Porcine Bone Morphogenetic Protein 15 (BMP15) ELISA Kit

The Porcine Bone Morphogenetic Protein 15 (BMP15) ELISA Kit is a highly reliable and accurate tool for the detection of BMP15 levels in porcine serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.BMP15 is a key protein involved in regulating folliculogenesis and ovulation in pigs, making it a valuable biomarker for studying reproductive health and fertility in porcine species.

Research on BMP15 can provide valuable insights into reproductive disorders, as well as assist in the development of potential therapies for improving reproductive outcomes in pigs.Overall, the Porcine BMP15 ELISA Kit is an essential tool for researchers looking to investigate the role of BMP15 in porcine reproductive biology and develop advancements in the field of porcine fertility research.

Product Name:Porcine Bone Morphogenetic Protein 15 (BMP15) ELISA Kit
Product Code:PRDL00018
Size:96 Assays
Target:BMP15
Synonyms:GDF9B, GDF9-B, ODG2, POF4, Growth/differentiation factor 9B
Detection Method:Sandwich
Range:31.2-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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