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Porcine Atrial Natriuretic Peptide (ANP) ELISA Kit

SKU:
PRDL00011
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P24259
Sensitivity:
9.8pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
ANH,ANF,CDD,Atrial Natriuretic Factor,Atrial Natriuretic Hormone,Atriopeptin,Cardionatrine,Cardiodilatin
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
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Description

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Porcine Atrial Natriuretic Peptide (ANP) ELISA Kit

The Porcine Atrial Natriuretic Peptide (ANP) ELISA Kit offered by AssayGenie is a highly sensitive and specific assay designed for the precise detection of ANP levels in porcine serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.ANP is a key peptide involved in regulating blood pressure and fluid balance in the body. Elevated ANP levels are often associated with heart failure and other cardiovascular conditions, making it a valuable biomarker for studying these diseases and developing new treatment strategies.

With the AssayGenie Porcine ANP ELISA Kit, researchers can accurately measure ANP levels in porcine samples, enabling them to further understand the role of this important peptide in cardiovascular health and disease. Trust AssayGenie for high-quality, dependable ELISA kits for all your research needs.

Product Name:Porcine Atrial Natriuretic Peptide (ANP) ELISA Kit
Product Code:PRDL00011
Size:96 Assays
Target:ANP
Synonyms:ANH, ANF, CDD, Atrial Natriuretic Factor, Atrial Natriuretic Hormone, Atriopeptin, Cardionatrine, Cardiodilatin
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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