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Porcine Apolipoprotein A2 (APOA2) ELISA Kit

SKU:
PRDL00013
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
F1S1A9
Sensitivity:
1.2?g/mL
Range:
4.687-300?g/mL
ELISA Type:
Sandwich
Synonyms:
Apo-A2,Apolipoprotein A-II,ProapoA-II,Truncated apolipoprotein A-II
Reactivity:
Porcine
Sample Type:
Serum, Plasma And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Porcine Apolipoprotein A2 (APOA2) ELISA Kit

The Porcine Apolipoprotein A2 (APOA2) ELISA Kit is a specialized assay designed for the precise measurement of APOA2 levels in porcine serum, plasma, and tissue homogenates. This ELISA kit offers superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Apolipoprotein A2 is a key protein involved in lipid metabolism and cardiovascular health.

It plays a crucial role in regulating cholesterol levels and lipid transport, making it a valuable biomarker for studying cardiovascular diseases and metabolic disorders. By accurately quantifying APOA2 levels, researchers can gain insights into the pathways and mechanisms underlying these conditions, potentially leading to the development of novel therapeutic strategies.

Product Name:Porcine Apolipoprotein A2 (APOA2) ELISA Kit
Product Code:PRDL00013
Size:96 Assays
Target:APOA2
Synonyms:Apo-A2, Apolipoprotein A-II, ProapoA-II, Truncated apolipoprotein A-II
Detection Method:Sandwich
Range:4.687-300?g/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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