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Porcine Anti-Mullerian Hormone (AMH) ELISA Kit

SKU:
PRDL00008
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P79295
Sensitivity:
0.041ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MIF,MIH,MIS,M?ºllerian Inhibiting Factor,M?ºllerian Inhibiting Hormone,M?ºllerian Inhibiting Substance
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Porcine Anti-Mullerian Hormone (AMH) ELISA Kit

The Porcine Anti-Mullerian Hormone (AMH) ELISA Kit offered by Assay Genie is specifically designed for the accurate detection of AMH levels in porcine serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.AMH is a key hormone involved in reproductive biology, playing a crucial role in the regulation of ovarian follicle development and fertility in pigs.

This biomarker has significant implications in studies related to reproductive disorders, fertility treatments, and ovarian function, making the Porcine AMH ELISA Kit an essential tool for researchers in this field.With its user-friendly protocol and reliable performance, the Porcine Anti-Mullerian Hormone ELISA Kit from Assay Genie is the ideal choice for researchers looking to accurately measure and study AMH levels in porcine samples.

Product Name:Porcine Anti-Mullerian Hormone (AMH) ELISA Kit
Product Code:PRDL00008
Size:96 Assays
Target:AMH
Synonyms:MIF, MIH, MIS, Müllerian Inhibiting Factor, Müllerian Inhibiting Hormone, Müllerian Inhibiting Substance
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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