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Porcine Angiopoietin 2 (ANGPT2) ELISA Kit

SKU:
PRDL00010
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9BDY7
Sensitivity:
0.54ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
ANG2,AGPT2
Reactivity:
Porcine
Sample Type:
Serum, Plasma And Other Biological Fluids
$899
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Description

system_update_altDatasheet

Porcine Angiopoietin 2 (ANGPT2) ELISA Kit

The Porcine ANGPT2 (Angiopoietin-2) ELISA Kit is specifically designed for the precise measurement of Angiopoietin-2 levels in porcine serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Angiopoietin-2 is a key player in angiogenesis, regulating blood vessel formation and cell growth.

Its involvement in various diseases such as cancer, cardiovascular disorders, and neurodegenerative conditions underscores its importance as a biomarker for disease monitoring and therapeutic development.Click the link below to learn more and purchase the Porcine Angiopoietin-2 ELISA Kit:[Link to product page](www.assaygenie.com/porcine-angiopoietin-2-angpt2-elisa-kit-1/)

Product Name:Porcine Angiopoietin 2 (ANGPT2) ELISA Kit
Product Code:PRDL00010
Size:96 Assays
Target:ANGPT2
Synonyms:ANG2, AGPT2
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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