null

Porcine Angiopoietin 1 (ANGPT1) ELISA Kit

SKU:
PRDL00009
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9BDY8
Sensitivity:
0.58ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
ANG1,AGP1,AGPT,ANG-I
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Porcine Angiopoietin 1 (ANGPT1) ELISA Kit

The Porcine ANGPT1 (Angiopoietin 1) ELISA Kit is a cutting-edge tool for detecting Angiopoietin 1 levels in porcine samples including serum, plasma, and cell culture supernatants. With superior sensitivity and specificity, this kit provides reliable and consistent results, making it a versatile option for a variety of research purposes.Angiopoietin 1 is a key protein involved in angiogenesis, the process of blood vessel formation, and plays a crucial role in regulating cell proliferation.

Its implications in various health conditions such as cancer, cardiovascular diseases, and neurodegenerative disorders make it a valuable biomarker for studying these diseases and potentially developing effective treatments.Order the Porcine ANGPT1 ELISA Kit today to advance your research and gain valuable insights into the role of Angiopoietin 1 in porcine physiology and disease pathology.

Product Name:Porcine Angiopoietin 1 (ANGPT1) ELISA Kit
Product Code:PRDL00009
Size:96 Assays
Target:ANGPT1
Synonyms:ANG1, AGP1, AGPT, ANG-I
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products