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Porcine Alpha-2-Heremans Schmid Glycoprotein (aHSG) ELISA Kit

SKU:
PRDL00006
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P29700
Sensitivity:
6.7ng/mL
Range:
15.625-1000ng/mL
ELISA Type:
Sandwich
Synonyms:
HSGa,A2HS,AHS,FETUA,HSG-A2,Fetuin-A,Alpha 2-HS Glycoprotein,Fetuin A,Alpha-2-Z-Globulin,Ba-alpha-2-Glycoprotein
Reactivity:
Porcine
Sample Type:
Serum, Plasma And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Porcine Alpha-2-Heremans Schmid Glycoprotein (aHSG) ELISA Kit

The Porcine Alpha-2 Heremans-Schmid Glycoprotein (AHSG) ELISA Kit is specially designed for precise and reliable detection of AHSG levels in porcine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees accurate and reproducible results, making it the perfect choice for various research purposes.AHSG, also known as Fetuin- A, is a key glycoprotein that plays a crucial role in various biological processes such as inflammation, insulin resistance, and bone metabolism.

Its levels have been associated with conditions like diabetes, cardiovascular diseases, and liver disorders, making it a valuable biomarker for studying these conditions and exploring potential treatment options.Don't miss out on the opportunity to unravel the secrets of AHSG with the advanced Porcine Alpha-2 Heremans-Schmid Glycoprotein ELISA Kit and take your research to the next level.

Product Name:Porcine Alpha-2-Heremans Schmid Glycoprotein (aHSG) ELISA Kit
Product Code:PRDL00006
Size:96 Assays
Target:aHSG
Synonyms:HSGa, A2HS, AHS, FETUA, HSG-A2, Fetuin-A, Alpha 2-HS Glycoprotein, Fetuin A, Alpha-2-Z-Globulin, Ba-alpha-2-Glycoprotein
Detection Method:Sandwich
Range:15.6-1000ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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