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Porcine Alanine Aminotransferase (ALT) ELISA Kit

SKU:
PRDL00007
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P13191
Sensitivity:
1.17U/L
Range:
4.687-300U/L
ELISA Type:
Sandwich
Synonyms:
GPT,ALAT,SGPT,AAT1,GPT1,Serum Glutamic Pyruvic Transaminase,Alanine Transaminase,Glutamate pyruvate transaminase 1,Glutamic--alanine transaminase 1
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Porcine Alanine Aminotransferase (ALT) ELISA Kit

The Porcine Alanine Aminotransferase (ALT) ELISA Kit from Assay Genie is a cutting-edge tool for the precise measurement of ALT levels in porcine serum, plasma, and tissues. With superior sensitivity and specificity, this kit delivers dependable and consistent results, making it an invaluable asset for a wide array of research studies.ALT is a key enzyme involved in amino acid metabolism and is primarily found in the liver. Elevated levels of ALT in blood can indicate liver damage or disease, making it a crucial biomarker for monitoring liver health in pigs.

This ELISA kit enables researchers to accurately quantify ALT levels in porcine samples, providing valuable insights into liver function and potential liver-related disorders.Whether studying liver diseases, evaluating drug toxicity, or monitoring animal health, the Porcine ALT ELISA Kit offers a comprehensive solution for researchers seeking reliable and precise measurements of ALT levels in porcine samples. Accelerate your research with the Assay Genie Porcine ALT ELISA Kit today.

Product Name:Porcine Alanine Aminotransferase (ALT) ELISA Kit
Product Code:PRDL00007
Size:96 Assays
Target:ALT
Synonyms:GPT, ALAT, SGPT, AAT1, GPT1, Serum Glutamic Pyruvic Transaminase, Alanine Transaminase, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1
Detection Method:Sandwich
Range:4.687-300U/L
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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