The POP4 Polyclonal Antibody (CAB12563) is a highly specific research tool for studying POP4, a key protein involved in RNA processing and ribosome biogenesis. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By targeting the POP4 protein, researchers can effectively detect and analyze its expression in various cell types, making it an essential tool for investigations in molecular biology and gene regulation.POP4, a component of the RNase P complex, plays a crucial role in cleaving precursor tRNAs to generate mature tRNAs, essential for protein synthesis.
Its involvement in ribosome biogenesis highlights its importance in cellular processes and makes it an intriguing target for studying RNA metabolism and gene expression regulation. The POP4 Polyclonal Antibody enables researchers to delve deeper into the functions of POP4 and explore its implications in various biological pathways, shedding light on potential therapeutic targets in diseases related to RNA processing and protein synthesis.
Product Name:
POP4 Rabbit Polyclonal Antibody
SKU:
CAB12563
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human POP4 (NP_006618.1).
This gene encodes one of the protein subunits of the small nucleolar ribonucleoprotein complexes: the endoribonuclease for mitochondrial RNA processing complex and the ribonuclease P complex. The encoded protein is localized to the nucleus and associates directly with the RNA component of these complexes. This protein is involved in processing of precursor RNAs. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
10775
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using POP4 Antibody (CAB12563) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
