The POMT1 Polyclonal Antibody (CAB10281) is a valuable tool for researchers studying POMT1, a critical enzyme involved in protein glycosylation and muscular dystrophy. This antibody, produced in rabbits, is highly specific to human samples and is suitable for use in Western blotting and immunohistochemistry applications. By targeting the POMT1 protein, researchers can easily detect and analyze its expression in various tissue types, making it an ideal choice for studies in developmental biology and neuromuscular disorders.POMT1 is essential for the proper glycosylation of proteins in the endoplasmic reticulum and Golgi apparatus, playing a key role in muscle development and function.
Mutations in the POMT1 gene are associated with various forms of muscular dystrophy, highlighting the importance of understanding its function and regulation. By studying POMT1 expression and activity, researchers can gain insights into the molecular mechanisms underlying muscular dystrophy and potentially identify new therapeutic targets for treating these debilitating conditions.
Product Name:
POMT1 Rabbit Polyclonal Antibody
SKU:
CAB10281
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 310-550 of human POMT1 (NP_001070833.1).
The protein encoded by this gene is an O-mannosyltransferase that requires interaction with the product of the POMT2 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of Walker-Warburg syndrome (WWS) and limb-girdle muscular dystrophy type 2K (LGMD2K). Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10585
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using POMT1 Rabbit pAb (CAB10281) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
