The POMK Polyclonal Antibody (CAB15529) is a key tool for researchers studying POMK, a protein involved in glycosylation and muscular dystrophy. This antibody, generated through rabbit immunization, exhibits high specificity for human samples and is validated for use in Western blot applications. By binding to the POMK protein, this antibody allows for precise detection and analysis in various cell types, making it an excellent choice for investigations in glycobiology and neuromuscular disorders.
POMK is essential for the glycosylation of alpha-dystroglycan, a process critical for maintaining muscle integrity and function. Dysregulation of POMK has been implicated in various forms of muscular dystrophy, making it a promising target for therapeutic interventions. Understanding the role of POMK in glycosylation pathways can provide valuable insights for the development of treatments for muscular dystrophies and other related conditions.
Product Name:
POMK Rabbit Polyclonal Antibody
SKU:
CAB15529
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human POMK (NP_115613.1).
Endoplasmic reticulum membrane, Single-pass type II membrane protein.
Calculated MW:
40kDa
Observed MW:
45kDa
This gene encodes a protein that may be involved in the presentation of the laminin-binding O-linked carbohydrate chain of alpha-dystroglycan (a-DG), which forms transmembrane linkages between the extracellular matrix and the exoskeleton. Some pathogens use this O-linked carbohydrate unit for host entry. Loss of function compound heterozygous mutations in this gene were found in a human patient affected by the Walker-Warburg syndrome (WWS) phenotype. Mice lacking this gene contain misplaced neurons (heterotopia) in some regions of the brain, possibly from defects in neuronal migration. Alternative splicing of this gene results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
84197
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using POMK Rabbit pAb (CAB15529) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
