The POMC Polyclonal Antibody (CAB3038) is a valuable tool for researchers studying the proopiomelanocortin (POMC) protein, which serves as a precursor for several important peptides involved in regulating various physiological processes. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting POMC in human samples, making it ideal for use in experiments such as Western blotting.POMC is known to play a critical role in functions such as energy homeostasis, stress response, and immune regulation, making it a key target for investigations into conditions like obesity, stress-related disorders, and autoimmune diseases.
By enabling the accurate detection and analysis of POMC in different cell types, the CAB3038 antibody facilitates the study of POMC's involvement in these processes, ultimately contributing to a better understanding of its potential therapeutic implications.Overall, the POMC Polyclonal Antibody (CAB3038) offers researchers a reliable tool for exploring the complex roles of POMC in various biological pathways, paving the way for advancements in the fields of metabolism, neurobiology, and immunology.
Product Name:
POMC Rabbit Polyclonal Antibody
SKU:
CAB3038
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human POMC (NP_000930.1).
This gene encodes a preproprotein that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the preproprotein and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.
Purification Method:
Affinity purification
Gene ID:
5443
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from 293F-POMC-His-C cells, using POMC Rabbit pAb (CAB3038) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
