Anti-Ubiquitin Antibody (CAB2129)
- SKU:
- CAB2129
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Anti-Ubiquitin Antibody (CAB2129)
The Polyubiquitin B Polyclonal Antibody (CAB2129) is a valuable tool for research involving polyubiquitin B, a crucial protein involved in the regulation of cellular processes through protein degradation. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blotting applications. By targeting polyubiquitin B, researchers can detect and analyze this protein in a variety of cell types, making it an ideal choice for studies in cell biology and molecular biology.Polyubiquitin B is a key player in the ubiquitin-proteasome system, a major pathway for protein degradation in cells.
Dysregulation of this system has been implicated in various diseases, including neurodegenerative disorders and cancer. Therefore, understanding the function and regulation of polyubiquitin B is essential for elucidating disease mechanisms and developing targeted therapies.Overall, the Polyubiquitin B Polyclonal Antibody (CAB2129) serves as a reliable tool for researchers investigating the role of polyubiquitin B in cellular physiology and disease pathogenesis. Its specificity and sensitivity make it a valuable asset in advancing our understanding of protein degradation pathways and identifying potential therapeutic targets.
| Antibody Name: | Anti-Ubiquitin Antibody |
| Antibody SKU: | CAB2129 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-76 of human Ubiquitin (NP_061828.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | BT-474, SW480, Mouse kidney, Mouse liver, Rat brain, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-76 of human Ubiquitin (NP_061828.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MQIF VKTL TGKT ITLE VEPS DTIE NVKA KIQD KEGI PPDQ QRLI FAGK QLED GRTL SDYN IQKE STLH LVLR LRGG |
| Gene ID: | 7314 |
| Uniprot: | P0CG47 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 25kDa |
| Observed MW: | 17-110kDa |
| Synonyms: | HEL-S-50, Ubiquitin, UBB |
| Background: | This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | UBB: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Belongs to the ubiquitin family. |
| UniProt Protein Details: | Protein type:Apoptosis; Ubiquitin-like modifier; Cell development/differentiation; Cell cycle regulation; Transcription regulation; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 17p12-p11.2 Cellular Component: nucleoplasm; neuron projection; cell soma; mitochondrion; plasma membrane; endosome membrane; cytosol Molecular Function:protein binding Biological Process: circadian rhythm; I-kappaB kinase/NF-kappaB cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of apoptosis; activation of MAPK activity; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; endosome transport; T cell receptor signaling pathway; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; activation of NF-kappaB transcription factor; mitochondrion transport along microtubule; regulation of apoptosis; toll-like receptor 5 signaling pathway; antigen processing and presentation of peptide antigen via MHC class I; regulation of mitochondrial membrane potential; transforming growth factor beta receptor signaling pathway; JNK cascade; antigen processing and presentation of exogenous peptide antigen via MHC class I; G2/M transition of mitotic cell cycle; toll-like receptor 4 signaling pathway; regulation of interferon type I production; glycogen biosynthetic process; fibroblast growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; glucose metabolic process; Notch receptor processing; virus assembly; toll-like receptor 2 signaling pathway; carbohydrate metabolic process; viral protein processing; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of interferon type I production; negative regulation of apoptosis; G1/S transition of mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of epidermal growth factor receptor signaling pathway; apoptosis; pathogenesis; negative regulation of transcription from RNA polymerase II promoter; viral infectious cycle; toll-like receptor 10 signaling pathway; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of interferon type I production; transmembrane transport; epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; DNA repair; MyD88-dependent toll-like receptor signaling pathway; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; toll-like receptor signaling pathway; innate immune response; gene expression; mitotic cell cycle; negative regulation of transforming growth factor beta receptor signaling pathway; neurite morphogenesis Disease: Cleft Palate, Isolated |
| NCBI Summary: | This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
| UniProt Code: | P0CG47 |
| NCBI GenInfo Identifier: | 302595875 |
| NCBI Gene ID: | 7314 |
| NCBI Accession: | P0CG47.1 |
| UniProt Secondary Accession: | P0CG47,P0CG48, P62979, P62987, |
| UniProt Related Accession: | P0CG47 |
| Molecular Weight: | |
| NCBI Full Name: | Polyubiquitin-B |
| NCBI Synonym Full Names: | ubiquitin B |
| NCBI Official Symbol: | UBB |
| NCBI Official Synonym Symbols: | HEL-S-50 |
| NCBI Protein Information: | polyubiquitin-B |
| UniProt Protein Name: | Polyubiquitin-B |
| Protein Family: | Ubiquilin |
| UniProt Gene Name: | UBB |
| UniProt Entry Name: | UBB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Ubiquitin antibody (CAB2129) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded Human colon using Ubiquitin Rabbit pAb (CAB2129) at dilution of 1:100 (40x lens). |
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