Anti-POLRMT Antibody (CAB15605)

Antibody Name:	Anti-POLRMT Antibody
Antibody SKU:	CAB15605
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 42-230 of human POLRMT (NP_005026.3).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Rat
Positive Samples:	HeLa, HepG2, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 42-230 of human POLRMT (NP_005026.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SSAS PQEQ DQDR RKDW GHVE LLEV LQAR VRQL QAES VSEV VVNR VDVA RLPE CGSG DGSL QPPR KVQM GAKD ATPV PCGR WAKI LEKD KRTQ QMRM QRLK AKLQ MPFQ SGEF KALT RRLQ VEPR LLSK QMAG CLED CTRQ APES PWEE QLAR LLQE APGK LSLD VEQA PSGQ HSQA QLSG QQQR LLAF F
Gene ID:	5442
Uniprot:	O00411
Cellular Location:	Mitochondrion
Calculated MW:	138kDa
Observed MW:	139kDa

Synonyms:

POLRMT, APOLMT, MTRNAP, MTRPOL, h-mtRPOL

Background:

This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes.

UniProt Protein Function:	DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.
NCBI Summary:	This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. [provided by RefSeq, Jul 2008]
UniProt Code:	O00411
NCBI GenInfo Identifier:	110618253
NCBI Gene ID:	5442
NCBI Accession:	NP_005026.3
UniProt Secondary Accession:	O00411,O60370,
UniProt Related Accession:	O00411
Molecular Weight:	135
NCBI Full Name:	DNA-directed RNA polymerase, mitochondrial
NCBI Synonym Full Names:	RNA polymerase mitochondrial
NCBI Official Symbol:	POLRMT
NCBI Official Synonym Symbols:	APOLMT; MTRNAP; MTRPOL; h-mtRPOL
NCBI Protein Information:	DNA-directed RNA polymerase, mitochondrial
UniProt Protein Name:	DNA-directed RNA polymerase, mitochondrial
UniProt Gene Name:	POLRMT

	Western blot analysis of extracts of various cell lines, using POLRMT antibody (CAB15605) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of C6 cells using POLRMT antibody (CAB15605) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using POLRMT antibody (CAB15605) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug POLRMT antibody (CAB15605). Western blot was performed from the immunoprecipitate using POLRMT antibody (CAB15605) at a dilition of 1:1000.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)