The POLRMT Polyclonal Antibody (CAB15605) is a valuable tool for researchers studying POLRMT, a key enzyme involved in mitochondrial gene transcription. This antibody is produced in rabbits and has been validated for use in Western blot applications with human samples. By binding to the POLRMT protein, this antibody allows for the detection and analysis of POLRMT in various cell types, making it an excellent choice for studies in molecular biology and mitochondrial research.POLRMT is essential for the transcription of mitochondrial DNA and plays a crucial role in mitochondrial gene expression and function.
Dysregulation of POLRMT has been implicated in a range of diseases, including mitochondrial disorders and cancer. By studying POLRMT, researchers can gain valuable insights into the molecular mechanisms underlying these conditions and potentially identify new therapeutic targets for treatment.Overall, the POLRMT Polyclonal Antibody is a high-quality reagent that enables precise and accurate analysis of POLRMT expression, making it a valuable asset for researchers investigating mitochondrial biology and its role in human health and disease.
Product Name:
POLRMT Rabbit Polyclonal Antibody
SKU:
CAB15605
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 42-230 of human POLRMT (NP_005026.3).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
APOLMT; MTRNAP; MTRPOL; COXPD55; h-mtRPOL; POLRMT
Positive Sample:
HeLa,HepG2,293T
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion.
Calculated MW:
139kDa
Observed MW:
139kDa
This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes.
Purification Method:
Affinity purification
Gene ID:
5442
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using POLRMT Rabbit pAb (CAB15605) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
