The POLR3K Polyclonal Antibody (CAB6467) is a valuable tool for researchers studying POLR3K, a subunit of RNA polymerase III involved in transcription. This antibody, produced in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. It specifically binds to the POLR3K protein, allowing for the detection and analysis of POLR3K expression in various cell types.POLR3K is a key component of the RNA polymerase III complex, essential for the transcription of small RNAs. Dysregulation of POLR3K has been linked to various diseases, including cancer and neurodevelopmental disorders.
By targeting POLR3K, researchers can gain insights into the molecular mechanisms underlying these conditions, paving the way for potential therapeutic interventions.Overall, the POLR3K Polyclonal Antibody (CAB6467) is a valuable tool for investigating the role of POLR3K in transcriptional regulation and its implications in disease pathology. Its high specificity and sensitivity make it ideal for a wide range of research applications in molecular biology and biomedical sciences.
Product Name:
POLR3K Rabbit Polyclonal Antibody
SKU:
CAB6467
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human POLR3K (NP_057394.3).
This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17.
Purification Method:
Affinity purification
Gene ID:
51728
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using POLR3K antibody (CAB6467) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
