The POLR3H Polyclonal Antibody (PACO22315) is a valuable tool for researchers studying POLR3H, a critical subunit of RNA polymerase III. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the POLR3H protein, this antibody allows for the detection and analysis of POLR3H in a variety of cell types, making it ideal for studies in molecular biology and gene expression regulation.POLR3H is essential for the transcription of small non-coding RNAs, playing a key role in processes such as ribosome biogenesis and mRNA translation.
Dysregulation of POLR3H has been implicated in various diseases, including cancer and developmental disorders. Understanding the functions of POLR3H is crucial for elucidating its role in these conditions and may lead to the development of targeted therapies.Overall, the POLR3H Polyclonal Antibody is a valuable tool for researchers investigating the role of POLR3H in gene expression and disease pathogenesis. Its high specificity and reactivity make it a reliable reagent for various experimental applications.
Antibody Name:
POLR3H Antibody (PACO22315)
Antibody SKU:
PACO22315
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from internal of human RPC8.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from K562 cells, using RPC8 antibody.
Background:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway By similarity.
Synonyms:
DNA-directed RNA polymerase III subunit RPC8; RNA polymerase III subunit C8; DNA-directed RNA polymerase III subunit H; DNA-directed RNA polymerase III subunit 22.9 kDa polypeptide; POLR3H
UniProt Protein Function:
POLR3H: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 22q13.2Cellular Component: centrosome; cytosol; DNA-directed RNA polymerase III complex; intracellular membrane-bound organelle; nucleoplasmMolecular Function: DNA binding; DNA-directed RNA polymerase activityBiological Process: defense response to virus; gene expression; innate immune response; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; positive regulation of interferon type I production; RNA elongation from RNA polymerase III promoter; termination of RNA polymerase III transcription; transcription from RNA polymerase III promoter; transcription initiation from RNA polymerase III promoter
