The POLR3H Polyclonal Antibody (PACO02585) is a valuable tool for researchers investigating POLR3H, a subunit of RNA polymerase III involved in the transcription of various non-coding RNAs. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By specifically binding to the POLR3H protein, this antibody enables the detection and analysis of POLR3H expression in a variety of cell types, making it an ideal choice for studies in molecular biology and gene regulation.POLR3H plays a crucial role in the transcription of a wide range of non-coding RNAs, including transfer RNAs and small nuclear RNAs, essential for cellular functions such as protein synthesis and RNA processing.
Dysregulation of POLR3H expression has been implicated in various diseases, including neurodevelopmental disorders and cancer, highlighting the importance of studying its function. The POLR3H Polyclonal Antibody provides researchers with a reliable tool to explore the role of POLR3H in health and disease, paving the way for potential therapeutic interventions in the future.
Antibody Name:
POLR3H Antibody
Antibody SKU:
PACO02585
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB, IHC
Recommended Dilutions:
WB:1:500-1:2000, IHC:1:100-1:300
Species Reactivity:
Human, Mouse, Rat
Immunogen:
synthesized peptide derived from the C-terminal region of human POLR3H.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
POLR3H; KIAA1665; RPC8; DNA-directed RNA polymerase III subunit RPC8; RNA polymerase III subunit C8; DNA-directed RNA polymerase III subunit H; RNA polymerase III subunit 22.9 kDa subunit; RPC22.9
UniProt Protein Function:
POLR3H: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 22q13.2Cellular Component: centrosome; cytosol; DNA-directed RNA polymerase III complex; intracellular membrane-bound organelle; nucleoplasmMolecular Function: DNA binding; DNA-directed RNA polymerase activityBiological Process: defense response to virus; gene expression; innate immune response; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; positive regulation of interferon type I production; RNA elongation from RNA polymerase III promoter; termination of RNA polymerase III transcription; transcription from RNA polymerase III promoter; transcription initiation from RNA polymerase III promoter
