The POLR2M Polyclonal Antibody (PAC05454) is a vital tool for researchers investigating POLR2M, an essential component of the RNA polymerase II complex involved in transcription. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it suitable for Western blot applications. By binding specifically to the POLR2M protein, this antibody allows for precise detection and analysis in a variety of cell types, making it a valuable resource for studies in molecular biology and gene expression regulation.
POLR2M is crucial for the transcription process, playing a key role in the synthesis of mRNA molecules. As such, understanding the function and regulation of POLR2M is vital for unraveling the complexities of gene expression and RNA processing. Research into POLR2M has implications for various fields, including molecular biology, genetics, and developmental biology, making the POLR2M Polyclonal Antibody (PAC05454) an indispensable tool for scientists seeking to advance knowledge in these areas.
Antibody Name:
POLR2M Antibody (PACO54454)
Antibody SKU:
PACO54454
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human DNA-directed RNA polymerase II subunit GRINL1A, isoforms 4/5 protein (2-91AA)
Immunofluorescent analysis of A549 cells using PACO54454 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Synonyms:
DNA-directed RNA polymerase II subunit GRINL1A, isoforms 4/5 (DNA-directed RNA polymerase II subunit M, isoforms 4/5), POLR2M, GRINL1A
UniProt Protein Function:
Isoform 1 appears to be a stable component of the Pol II(G) complex form of RNA polymerase II (Pol II). Pol II synthesizes mRNA precursors and many functional non-coding RNAs and is the central component of the basal RNA polymerase II transcription machinery. Isoform 1 may play a role in the Mediator complex-dependent regulation of transcription activation. Isoform 1 acts in vitro as a negative regulator of transcriptional activation; this repression is relieved by the Mediator complex, which restores Pol II(G) activator-dependent transcription to a level equivalent to that of Pol II.
NCBI Summary:
This gene encodes a subunit of a specific form of RNA polymerase II termed Pol II(G). The encoded protein may act as a negative regulator of transcriptional activation by the Mediator complex. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 4. Readthrough transcription between this gene and the neighboring upstream gene MYZAP (myocardial zonula adherens protein) is represented with GeneID 145781. [provided by RefSeq, Oct 2013]
