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POLR2J Rabbit Polyclonal Antibody (CAB13627)

SKU:
CAB13627
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-POLR2J Antibody CAB13627
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-POLR2J Antibody CAB13627
$167 - $503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:POLR2J Rabbit Polyclonal Antibody
SKU:CAB13627
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLR2J (NP_006225.1).
Sequence:MNAP PAFE SFLL FEGE KKIT INKD TKVP NACL FTIN KEDH TLGN IIKS QLLK DPQV LFAG YKVP HPLE HKII IRVQ TTPD YSPQ EAFT NAIT DLIS ELSL LEER FRVA IKDK QEGI E
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:RPB11; RPB11A; RPB11m; hRPB14; POLR2J1; POLR2J
Positive Sample:Mouse skeletal muscle
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:13kDa
Observed MW:13kDa

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.

Purification Method:Affinity purification
Gene ID:5439
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of mouse skeletal muscle, using POLR2J antibody (CAB13627) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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