The POLR2J Polyclonal Antibody (CAB13627) is a research tool designed for the study of POLR2J, a key component of the RNA polymerase II enzyme complex responsible for transcribing DNA into RNA. This antibody, generated in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the POLR2J protein, researchers can effectively detect and analyze its expression in various cell types, making it a valuable tool for investigations in molecular biology and gene expression studies.POLR2J is essential for the accurate transcription of genes and plays a crucial role in regulating gene expression in normal cellular processes.
Dysregulation of POLR2J has been linked to various diseases, including cancer and developmental disorders, highlighting its importance in understanding transcription mechanisms and gene regulation. With its ability to target POLR2J, this antibody provides researchers with the means to explore the role of this protein in different biological contexts and uncover potential therapeutic targets for treating associated conditions.
Product Name:
POLR2J Rabbit Polyclonal Antibody
SKU:
CAB13627
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLR2J (NP_006225.1).
This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.
Purification Method:
Affinity purification
Gene ID:
5439
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse skeletal muscle, using POLR2J antibody (CAB13627) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
