The POLR2I Polyclonal Antibody (CAB1865) is designed for research involving POLR2I, a subunit of RNA polymerase that plays a crucial role in transcription. The antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. It binds specifically to the POLR2I protein, allowing for accurate detection and analysis in a variety of cell types.POLR2I is essential for the transcription of protein-coding genes and is involved in various biological processes, including cell growth and differentiation.
Dysregulation of POLR2I activity has been linked to diseases such as cancer, making it a valuable target for research in oncology and molecular biology. Studying the function of POLR2I can provide insights into its role in disease development and progression, leading to potential therapeutic interventions targeting transcriptional dysregulation.
Product Name:
POLR2I Rabbit Polyclonal Antibody
SKU:
CAB1865
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human POLR2I (NP_006224.1).
Sequence:
MEPD GTYE PGFV GIRF CQEC NNML YPKE DKEN RILL YACR NCDY QQEA DNSC IYVN KITH EVDE LTQI IADV SQDP TLPR TEDH PCQK CGHK EAVF FQSH SARA EDAM RLYY VCTA PHCG HRWT E
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
RPB9; hRPB14.5; POLR2I
Positive Sample:
MCF7,SW480,HL-60,22Rv1
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, nucleolus.
Calculated MW:
15kDa
Observed MW:
15kDa
This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity.
Purification Method:
Affinity purification
Gene ID:
5438
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using POLR2I antibody (CAB1865) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
