The POLR2H Polyclonal Antibody (CAB6948) is a valuable tool for researchers studying POLR2H, a subunit of RNA polymerase II essential for transcription. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the POLR2H protein, this antibody allows for accurate detection and analysis in various cell types, making it ideal for investigations in molecular biology and gene expression studies.POLR2H is a crucial component of the RNA polymerase II complex, which is responsible for transcribing protein-coding genes in eukaryotic cells.
Understanding the function and regulation of POLR2H is essential for unraveling the complexities of gene expression and the mechanisms underlying various diseases, including cancer and developmental disorders. By targeting POLR2H, researchers can gain insights into transcriptional control and potentially identify new therapeutic targets for treating transcription-related diseases.
Product Name:
POLR2H Rabbit Polyclonal Antibody
SKU:
CAB6948
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human POLR2H (NP_006223.2).
The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene.
Purification Method:
Affinity purification
Gene ID:
5437
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using POLR2H Rabbit pAb (CAB6948) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
