The POLR2H Polyclonal Antibody (CAB13339) is a valuable tool for research involving POLR2H, a key subunit of RNA polymerase II that plays a crucial role in transcription. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the POLR2H protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in molecular biology and gene expression research.POLR2H is essential for the transcription of protein-coding genes and is involved in various cellular processes, making it a target of interest in studies related to gene regulation, RNA synthesis, and transcriptional control.
Understanding the function and regulation of POLR2H is key to unraveling the complexities of gene expression and may have implications in disease research, particularly in conditions related to aberrant transcriptional activity.By utilizing the POLR2H Polyclonal Antibody, researchers can gain valuable insights into the role of POLR2H in transcriptional processes, potentially leading to advancements in areas such as drug discovery, gene therapy, and understanding of genetic disorders. This antibody serves as a reliable tool for investigating the functions and mechanisms of POLR2H, opening up new possibilities for scientific exploration and discovery.
Product Name:
POLR2H Rabbit Polyclonal Antibody
SKU:
CAB13339
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human POLR2H (NP_006223.2).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
RPB8; RPB17; RPABC3; POLR2H
Positive Sample:
SW480,MCF7,HL-60,293T,Mouse spleen,Mouse thymus
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, nucleolus.
Calculated MW:
17kDa
Observed MW:
17kDa
The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene.
Purification Method:
Affinity purification
Gene ID:
5437
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using POLR2H Rabbit pAb (CAB13339) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
