The POLR2F Polyclonal Antibody (CAB6837) is specifically designed for research involving POLR2F, a subunit of RNA polymerase II essential for transcription. This antibody, generated in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By binding to the POLR2F protein, it allows for the detection and analysis of POLR2F in a variety of cell types, making it ideal for studies in molecular biology and gene regulation.POLR2F plays a crucial role in the transcription process, serving as a key component of the RNA polymerase II complex responsible for transcribing protein-coding genes. Understanding the function of POLR2F is essential for deciphering the mechanisms of gene expression and regulation.
Its involvement in transcription makes it a valuable target for research into various biological processes and diseases related to gene expression dysregulation.By utilizing the POLR2F Polyclonal Antibody, researchers can gain valuable insights into the role of POLR2F in transcriptional regulation and potentially uncover new therapeutic targets for diseases involving aberrant gene expression. This antibody is a valuable tool for advancing research in molecular biology, genetics, and disease mechanisms related to transcriptional control.
Product Name:
POLR2F Rabbit Polyclonal Antibody
SKU:
CAB6837
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human POLR2F (NP_068809.1).
This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5435
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from rat heart, using POLR2F Rabbit pAb (CAB6837) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
