The POLR2E Polyclonal Antibody (CAB1755) is a research tool specifically designed for studying POLR2E, a subunit of RNA polymerase II involved in gene transcription. This antibody, generated in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. By targeting the POLR2E protein, this antibody allows for the detection and analysis of POLR2E in a variety of cell types. This makes it suitable for research in molecular biology, genetics, and genomics.POLR2E is an essential component of the RNA polymerase II complex, playing a crucial role in transcribing protein-coding genes.
Understanding the function and regulation of POLR2E is important for elucidating the mechanisms underlying gene expression and its dysregulation in diseases such as cancer and genetic disorders. By using the POLR21 Polyclonal Antibody, researchers can investigate the role of POLR2E in transcriptional processes and its potential as a therapeutic target for various diseases.
Product Name:
POLR2E Rabbit Polyclonal Antibody
SKU:
CAB1755
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human POLR2E (NP_002686.2).
This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5434
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using POLR2E Rabbit pAb (CAB1755) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
