The POLR2D Polyclonal Antibody (CAB1859) is developed for researchers studying POLR2D, a subunit of RNA polymerase II enzyme complex that plays a key role in transcription. This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it suitable for Western blot applications. By binding to the POLR2D protein, researchers can detect and analyze its expression in various cell types, facilitating research in molecular biology and gene expression studies.
POLR2D is an essential component of the RNA polymerase II complex, involved in transcribing protein-coding genes and regulating gene expression. Understanding the function and regulation of POLR2D is crucial for unraveling the intricate mechanisms of transcription and gene regulation. Research on POLR2D can provide valuable insights into genetic disorders, developmental biology, and cancer biology, paving the way for novel therapeutic strategies targeting transcriptional dysregulation.
Product Name:
POLR2D Rabbit Polyclonal Antibody
SKU:
CAB1859
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human POLR2D (NP_004796.1).
WB,1:500 - 1:2000 IF/ICC,1:10 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
RBP4; RPB4; RPB16; HSRBP4; HSRPB4; POLR2D
Positive Sample:
MCF-7,SW480,SW620,HepG2,SK-HEPC,Mouse spleen
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
16kDa
Observed MW:
17kDa
This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene.
Purification Method:
Affinity purification
Gene ID:
5433
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using POLR2D Rabbit pAb (CAB1859) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
