The POLR2C Polyclonal Antibody (CAB1785) is a valuable tool for researchers investigating the POLR2C protein, a key component of RNA polymerase II that plays a crucial role in gene transcription. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the POLR2C protein, this antibody enables researchers to detect and analyze its expression in various cell types, making it an essential tool for studies in molecular biology and gene regulation.POLR2C is a subunit of RNA polymerase II, the enzyme responsible for transcribing mRNA in eukaryotic cells.
Its involvement in the transcription process makes it a vital target for research into gene expression, regulation, and the mechanisms underlying various diseases. By understanding the function and regulation of POLR2C, researchers can gain insights into how gene transcription is controlled and develop potential therapeutic interventions targeting this process. Explore the role of POLR2C in gene transcription and unlock new possibilities in the field of molecular biology with the POLR2C Polyclonal Antibody (CAB1785).
Product Name:
POLR2C Rabbit Polyclonal Antibody
SKU:
CAB1785
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human RPB3/POLR2C (NP_116558.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
RPB3; RPB31; hRPB33; hsRPB3; RPB3/POLR2C
Positive Sample:
SW620,OVCAR3,Mouse spleen
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
31kDa
Observed MW:
38kDa
This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21.
Purification Method:
Affinity purification
Gene ID:
5432
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using RPB3/POLR2C Rabbit pAb (CAB1785) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
