The POLR2B Polyclonal Antibody (CAB5928) is a valuable tool for researchers studying POLR2B, a subunit of RNA Polymerase II involved in transcription. This antibody, generated in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the POLR2B protein, this antibody allows for the detection and analysis of POLR2B in various cell types, making it ideal for studies in molecular biology and gene regulation research.POLR2B is a crucial component of the RNA Polymerase II complex, responsible for transcribing mRNA in eukaryotic cells. Understanding the function and regulation of POLR2B is essential for unraveling the complexities of gene expression and its role in various cellular processes.
Research into POLR2B is particularly relevant in fields such as transcriptional regulation, cancer biology, and developmental biology, where gene expression plays a critical role in disease progression and cellular development.Overall, the POLR2B Polyclonal Antibody provides researchers with a reliable tool for investigating the role of POLR2B in transcription and gene expression, offering insights into the molecular mechanisms underlying cellular processes and diseases. Its versatility and specificity make it a valuable asset for advancing research in molecular biology and related fields.
Product Name:
POLR2B Rabbit Polyclonal Antibody
SKU:
CAB5928
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2B (NP_000929.1).
This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5431
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using POLR2B Rabbit pAb (CAB5928) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
