The POLR2A Polyclonal Antibody (CAB2107) is a valuable tool for researchers studying POLR2A, a key component of the RNA polymerase II complex involved in transcription. This antibody, generated in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By specifically binding to the POLR2A protein, researchers can easily detect and analyze its expression in various cell types, making it ideal for studies in molecular biology, gene regulation, and transcriptional control.
POLR2A, also known as RNA polymerase II subunit A, is essential for the transcription of protein-coding genes and plays a crucial role in regulating gene expression. Dysfunction of POLR2A has been associated with various diseases, including cancer, developmental disorders, and metabolic conditions. By understanding the function and regulation of POLR2A, researchers can gain insights into the mechanisms underlying these diseases and potentially identify novel therapeutic targets for intervention.
Product Name:
POLR2A Rabbit Polyclonal Antibody
SKU:
CAB2107
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2A (NP_000928.1).
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Purification Method:
Affinity purification
Gene ID:
5430
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using POLR2A Rabbit pAb (CAB2107) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Negative control (NC): Human Plasma.Exposure time: 30s.
