POLR2A Rabbit Polyclonal Antibody (CAB2107)
- SKU:
- CAB2107
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
Product Name: | POLR2A Rabbit Polyclonal Antibody |
SKU: | CAB2107 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2A (NP_000928.1). |
Sequence: | MHGG GPPS GDSA CPLR TIKR VQFG VLSP DELK RMSV TEGG IKYP ETTE GGRP KLGG LMDP RQGV IERT GRCQ TCAG NMTE CPGH FGHI ELAK PVFH VGFL VKTM KVLR CVCF FCSK LLVD SNNP KIKD ILAK SKGQ PKKR LTHV YDLC KGKN ICEG GEEM DNKF GVEQ PEGD EDLT KEKG HGGC GRYQ PRIR RSGL ELYA EWKH VNED SQEK KILL SPER VHEI FKRI SDEE CFVL GMEP RYAR PEWM IVTV LPVP PLSV |
Tested Applications: | WB IF/ICC IP ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
Synonyms: | RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; NEDHIB; RpIILS; hsRPB1; hRPB220; POLR2A |
Positive Sample: | HeLa |
Conjugate: | Unconjugated |
Cellular Localization: | Nucleus. |
Calculated MW: | 217kDa |
Observed MW: | 210-250kDa |
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Purification Method: | Affinity purification |
Gene ID: | 5430 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |