The POLR1E Polyclonal Antibody (CAB12700) is a valuable tool for researchers studying POLR1E, a key subunit of RNA polymerase I involved in the transcription of ribosomal RNA. This antibody, raised in rabbits, exhibits high specificity and sensitivity in detecting POLR1E in human samples, making it ideal for Western blot applications.POLR1E plays a crucial role in ribosome biogenesis and protein synthesis, making it an essential component of cellular function. Dysregulation of POLR1E has been associated with various diseases, including cancer and developmental disorders.
Understanding the function and regulation of POLR1E is important for uncovering new therapeutic targets and developing treatments for these conditions.By using the POLR1E Polyclonal Antibody, researchers can explore the role of POLR1E in RNA transcription and its implications for disease pathology. This antibody enables precise detection and analysis of POLR1E expression in different cell types, providing valuable insights into its function and potential as a therapeutic target.
Product Name:
POLR1E Rabbit Polyclonal Antibody
SKU:
CAB12700
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human POLR1E (NP_071935.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
A49; PAF53; PRAF1; RPA49; POLR1E
Positive Sample:
HeLa,A-549,293T,Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, nucleolus.
Calculated MW:
47kDa
Observed MW:
53kDa
Predicted to enable DNA binding activity; DNA-directed 5'-3' RNA polymerase activity; and RNA polymerase I general transcription initiation factor binding activity. Involved in nucleolar large rRNA transcription by RNA polymerase I. Located in fibrillar center and nucleoplasm.
Purification Method:
Affinity purification
Gene ID:
64425
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using POLR1E Rabbit pAb (CAB12700) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
