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Anti-POLR1C Antibody (CAB0269)

SKU:
CAB0269
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-POLR1C Antibody CAB0269
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-POLR1C Antibody CAB0269
€139 - €419
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Description

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Antibody Name:Anti-POLR1C Antibody
Antibody SKU:CAB0269
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human POLR1C (NP_976035.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:K-562, 293T, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human POLR1C (NP_976035.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAS QAVE EMRS RVVL GEFG VRNV HTTD FPGN YSGY DDAW DQDR FEKN FRVD VVHM DENS LEFD MVGI DAAI ANAF RRIL LAEV PTMA VEKV LVYN NTSI VQDE ILAH RLGL IPIH ADPR LFEY RNQG DEEG TEID TLQF RLQV RCTR NPHA AKDS SDPN ELYV NHKV YTRH MTWI PLGN QADL FPEG TIRP VHDD ILIA
Gene ID:9533
Uniprot:O15160
Cellular Location:Nucleus
Calculated MW:38kDa/39kDa
Observed MW:39kDa
Synonyms:POLR1C, AC40, HLD11, RPA39, RPA40, RPA5, RPAC1, RPC40, TCS3
Background:The protein encoded by this gene is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Mutations in this gene have been associated with Treacher Collins syndrome (TCS) and hypomyelinating leukodystrophy 11. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:RPA40: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft. Defects in POLR1C are the cause of Treacher Collins syndrome type 3 (TCS3). A form of Treacher Collins syndrome, a disorder of craniofacial development. Treacher Collins syndrome is characterized by a combination of bilateral downward slanting of the palpebral fissures, colobomas of the lower eyelids with a paucity of eyelashes medial to the defect, hypoplasia of the facial bones, cleft palate, malformation of the external ears, atresia of the external auditory canals, and bilateral conductive hearing loss. Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transferase; EC 2.7.7.6; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Transcription initiation complex

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytosol; DNA-directed RNA polymerase I complex

Molecular Function:protein dimerization activity; DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; negative regulation of gene expression, epigenetic; positive regulation of interferon type I production; transcription from RNA polymerase I promoter; innate immune response; RNA elongation from RNA polymerase I promoter; gene expression; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; RNA elongation from RNA polymerase III promoter

Disease: Leukodystrophy, Hypomyelinating, 11; Treacher Collins Syndrome 3

NCBI Summary:The protein encoded by this gene is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Defects in this gene have been associated with Treacher Collins syndrome (TCS). [provided by RefSeq, Mar 2011]
UniProt Code:O15160
NCBI GenInfo Identifier:3914807
NCBI Gene ID:9533
NCBI Accession:O15160.1
UniProt Secondary Accession:O15160,O75395, Q5JTE3,
UniProt Related Accession:O15160
Molecular Weight:346
NCBI Full Name:DNA-directed RNA polymerases I and III subunit RPAC1
NCBI Synonym Full Names:polymerase (RNA) I polypeptide C, 30kDa
NCBI Official Symbol:POLR1C  
NCBI Official Synonym Symbols:RPA5; TCS3; RPA39; RPA40; RPAC1  
NCBI Protein Information:DNA-directed RNA polymerases I and III subunit RPAC1; AC40; RPC40; RNA polymerases I and III subunit AC1; DNA-directed RNA polymerase I subunit C; DNA-directed RNA polymerases I and III 40 kDa polypeptide
UniProt Protein Name:DNA-directed RNA polymerases I and III subunit RPAC1
UniProt Synonym Protein Names:AC40; DNA-directed RNA polymerases I and III 40 kDa polypeptide; RPA40; RPA39; RPC40
Protein Family:DNA-directed RNA polymerases
UniProt Gene Name:POLR1C  
UniProt Entry Name:RPAC1_HUMAN
Anti-POLR1C Antibody (CAB0269)Western blot analysis of extracts of various cell lines, using POLR1C antibody (CAB0269) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-POLR1C Antibody (CAB0269)Immunofluorescence analysis of HeLa cells using POLR1C antibody (CAB0269). Blue: DAPI for nuclear staining.

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