The POLR1B Polyclonal Antibody (CAB12034) is specifically designed for research involving POLR1B, a key enzyme that is essential for RNA transcription. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot and immunohistochemistry applications.POLR1B is a subunit of RNA polymerase I, which is responsible for transcribing ribosomal RNA genes. This enzyme plays a crucial role in the regulation of protein synthesis and cell growth. The POLR1B antibody enables detection and analysis of POLR1B protein levels in various cell types, making it an essential tool for studies in molecular biology, cell biology, and cancer research.
Understanding the function and regulation of POLR1B is important for unraveling the complexities of gene expression and discovering potential targets for therapeutic intervention. The POLR1B Polyclonal Antibody (CAB12034) provides researchers with a reliable tool to study the role of POLR1B in various cellular processes and diseases, leading to advancements in our understanding of RNA transcription and its implications in health and disease.
Product Name:
POLR1B Rabbit Polyclonal Antibody
SKU:
CAB12034
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 670-900 of human POLR1B (NP_061887.2).
Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).
Purification Method:
Affinity purification
Gene ID:
84172
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using POLR1B Rabbit pAb (CAB12034) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
