The POLL Polyclonal Antibody (CAB8408) is a vital tool for researchers studying the role of POLL, a protein involved in DNA repair processes and maintenance of genome stability. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been rigorously validated for use in Western blot applications. By specifically binding to POLL, this antibody allows for precise detection and analysis of the protein in various cell types, making it an excellent choice for studies in DNA repair mechanisms and cancer research.POLL, also known as DNA polymerase lambda, is essential for error-prone DNA repair pathways and plays a crucial role in maintaining genomic integrity.
Dysregulation of POLL activity has been linked to DNA damage accumulation and increased susceptibility to mutations, making it a potential target for therapies aimed at combating cancer and other genetic diseases. By delving into the function of POLL, researchers can uncover new insights into the mechanisms underlying DNA repair processes and develop targeted interventions for diseases characterized by genomic instability.
Product Name:
POLL Rabbit Polyclonal Antibody
SKU:
CAB8408
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 421-575 of human POLL (NP_037406.1).
This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described.
Purification Method:
Affinity purification
Gene ID:
27343
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using POLL antibody (CAB8408) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
