The POLG2 Polyclonal Antibody (CAB6695) is a valuable tool for researchers studying POLG2, a key enzyme involved in mitochondrial DNA replication. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications. By facilitating the detection and analysis of POLG2 protein expression in various cell types, this antibody is essential for advancing research in the fields of genetics, mitochondrial biology, and neurodegenerative diseases.POLG2 is an essential component of the DNA polymerase gamma complex, which is responsible for maintaining the integrity of mitochondrial DNA.
Mutations in the POLG2 gene have been linked to a range of mitochondrial disorders, including mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) and progressive external ophthalmoplegia (PEO). Understanding the function of POLG2 and its role in mitochondrial DNA replication is crucial for developing targeted therapies and interventions for these debilitating diseases. The POLG2 Polyclonal Antibody (CAB6695) offers researchers a reliable tool for studying POLG2 expression and function, ultimately contributing to advancements in the field of mitochondrial biology and genetics.
Product Name:
POLG2 Rabbit Polyclonal Antibody
SKU:
CAB6695
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 216-485 of human POLG2 (NP_009146.2).
This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions.
Purification Method:
Affinity purification
Gene ID:
11232
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using POLG2 Rabbit pAb (CAB6695) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
