DNA Polymerase gamma Rabbit Polyclonal Antibody (CAB8451)
The POLG Polyclonal Antibody (CAB8451) is a valuable tool for researchers studying the POLG protein, a key enzyme involved in mitochondrial DNA replication. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the POLG protein, researchers can effectively detect and analyze its expression in a variety of cell types.POLG is essential for maintaining mitochondrial DNA integrity and mutations in this gene have been associated with a range of diseases, including mitochondrial disorders and certain types of cancer.
By studying the function and regulation of POLG, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the POLG Polyclonal Antibody is a reliable tool for investigating the role of the POLG enzyme in health and disease. Its versatility and specificity make it an ideal choice for researchers in the fields of genetics, molecular biology, and mitochondrial biology.
Product Name:
DNA Polymerase gamma Rabbit Polyclonal Antibody
SKU:
CAB8451
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 540-720 of human DNA Polymerase gamma (NP_002684.1).
Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5428
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DNA Polymerase gamma Rabbit pAb antibody (CAB8451) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
