The Pole4 Polyclonal Antibody (CAB9882) is specifically designed for research involving the Pole4 protein, a key player in DNA replication and repair processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the Pole4 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an essential tool for studies in molecular biology and cancer research.Pole4, also known as DNA polymerase epsilon subunit 3, is essential for ensuring accurate DNA replication and maintaining genomic integrity.
Dysregulation of Pole4 has been implicated in various diseases, including cancer, where mutations in this protein can lead to genomic instability and tumor development. Research into the function of Pole4 is crucial for understanding its role in disease progression and developing targeted therapies for cancer treatment.Overall, the Pole4 Polyclonal Antibody (CAB9882) provides researchers with a reliable tool for studying the function and regulation of the Pole4 protein in various cellular processes, facilitating advancements in cancer research and drug development.
Product Name:
POLE4 Rabbit Polyclonal Antibody
SKU:
CAB9882
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLE4 (NP_063949.2).
POLE4 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.
Purification Method:
Affinity purification
Gene ID:
56655
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using POLE4 antibody (CAB9882) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
