The POLE4 Antibody (CAB9882) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the Pole4 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an essential tool for studies in molecular biology and cancer research.Pole4, also known as DNA polymerase epsilon subunit 3, is essential for ensuring accurate DNA replication and maintaining genomic integrity.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
POLE4 Antibody
SKU:
CAB9882
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p12, YHHQ1, POLE4
Positive Sample:
HeLa, 293T, HepG2, MCF-7, HL-60
Cellular Localization:
Nucleus.
Calculated MW:
12kDa
Observed MW:
17kDa
POLE4 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.
Purification Method
Affinity purification
Gene ID
56655
RRID
AB_2771737
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using POLE4 Rabbit pAb (CAB9882) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
