The Pole2 Polyclonal Antibody (CAB12842) is a specialized research tool designed for studying the protein Pole2, which plays a critical role in DNA replication and cell division. This antibody, produced in rabbits, is highly specific to Pole2 and is validated for use in Western blot applications. By binding to the Pole2 protein, researchers can detect and analyze its expression in a variety of cell types, making it an essential tool for studies in molecular biology and cancer research.Pole2 is an essential component of the DNA polymerase epsilon complex, which is responsible for DNA replication and repair. Its function is vital for maintaining genomic stability and preventing mutations that can lead to cancer and other genetic disorders.
By studying Pole2, researchers can gain insights into the mechanisms of DNA replication and the potential implications for disease development.Understanding the role of Pole2 in cellular processes is crucial for advancing our knowledge of cell division, DNA replication, and genomic stability. The Pole2 Polyclonal Antibody provides researchers with a reliable tool for investigating the functions of Pole2 and its potential implications for cancer research and other areas of biomedical science.
Product Name:
POLE2 Rabbit Polyclonal Antibody
SKU:
CAB12842
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human POLE2 (NP_002683.2).
DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. The protein encoded by this gene represents the small subunit (B). Defects in this gene have been linked to colorectal cancer and to combined immunodeficiency.
Purification Method:
Affinity purification
Gene ID:
5427
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using POLE2 Rabbit pAb (CAB12842) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
