The POLE Polyclonal Antibody (PACO22306) is a valuable tool for researchers studying POLE, a key enzyme involved in DNA replication and repair processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By specifically binding to the POLE protein, this antibody enables the detection and analysis of POLE expression in various cell types, making it an ideal choice for investigations in molecular biology and cancer research.POLE, also known as DNA polymerase epsilon, is essential for maintaining genomic stability and fidelity during cell division. Mutations in POLE have been linked to tumorigenesis and cancer progression, highlighting the importance of studying this enzyme in disease mechanisms.
By unraveling the mechanisms of POLE function, researchers can gain insights into potential therapeutic targets for cancer treatment and precision medicine approaches.Overall, the POLE Polyclonal Antibody (PACO22306) provides researchers with a powerful tool for investigating the role of POLE in DNA replication, repair, and genomic integrity. Its high specificity and sensitivity make it an indispensable reagent for advancing our understanding of fundamental biological processes and disease mechanisms.
Antibody Name:
POLE Antibody (PACO22306)
Antibody SKU:
PACO22306
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:50-1:100
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from internal of human POLE1.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using POLE1 antibody.
Background:
Participates in DNA repair and in chromosomal DNA replication.
Synonyms:
DNA polymerase epsilon catalytic subunit A; EC 2.7.7.7; DNA polymerase II subunit A;
UniProt Protein Function:
POLE: Participates in DNA repair and in chromosomal DNA replication. Belongs to the DNA polymerase type-B family.Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; EC 2.7.7.7; DNA replication; DNA repair, damage; TransferaseChromosomal Location of Human Ortholog: 12q24.3Cellular Component: nucleoplasm; epsilon DNA polymerase complex; cytoplasm; plasma membrane; nucleusMolecular Function: DNA binding; zinc ion binding; 4 iron, 4 sulfur cluster binding; nucleotide binding; DNA-directed DNA polymerase activity; chromatin bindingBiological Process: DNA replication initiation; in utero embryonic development; DNA repair; base-excision repair, gap-filling; telomere maintenance via semi-conservative replication; DNA synthesis during DNA repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycleDisease: Colorectal Cancer, Susceptibility To, 12; Facial Dysmorphism, Immunodeficiency, Livedo, And Short Stature
UniProt Protein Details:
NCBI Summary:
This gene encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature. [provided by RefSeq, Sep 2013]
