POLDIP3 Antibody (PACO55286)
- SKU:
- PACO55286
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- IP
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
POLDIP3 Antibody (PACO55286)
The POLDIP3 Polyclonal Antibody (PACO55286) is a valuable tool for researchers studying POLDIP3, a protein involved in DNA repair and replication processes. This antibody, produced in rabbits, shows high specificity for human samples and is suitable for use in Western blot applications. By binding to POLDIP3, researchers can detect and analyze the protein in various cell types, making it an ideal choice for studies in genetics and cancer research.POLDIP3, also known as polymerase delta interacting protein 3, plays a crucial role in maintaining genomic stability and preventing mutations during DNA replication.
Its involvement in DNA repair pathways and cell cycle regulation makes it a key player in understanding the mechanisms of cancer development and progression. By studying POLDIP3, researchers can gain insights into potential therapeutic targets for cancer treatment and improving genomic integrity.
| Antibody Name: | POLDIP3 Antibody (PACO55286) |
| Antibody SKU: | PACO55286 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF, IP |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:20-1:200, IP:1:200-1:2000, |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Polymerase delta-interacting protein 3 protein (100-221AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate. All lanes: POLDIP3 antibody at 3.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 43 kDa. Observed band size: 43 kDa. |
 | Immunofluorescence staining of Hela cells with PACO55286 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunohistochemistry of paraffin-embedded human testis tissue using PACO55286 at dilution of 1:100. |
| Background: | Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Involved in nuclear mRNA export; probably mediated by assoociation with the TREX complex. |
| Synonyms: | Polymerase delta-interacting protein 3 (46 kDa DNA polymerase delta interaction protein) (p46) (S6K1 Aly/REF-like target) (SKAR), POLDIP3, KIAA1649 PDIP46 |
| UniProt Protein Function: | SKAR: Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Interacts with POLD2. Interacts with NCBP1 and EIF4A3. Associates with the multiprotein exon junction complex (EJC). Interacts with RPS6KB1 (activated). Interacts with ERH. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:RNA-binding Chromosomal Location of Human Ortholog: 22q13.2 Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nuclear speck Molecular Function:protein binding; nucleotide binding Biological Process: poly(A)+ mRNA export from nucleus; positive regulation of translation |
| NCBI Summary: | This gene encodes an RRM (RNA recognition motif)-containing protein that participates in the regulation of translation by recruiting ribosomal protein S6 kinase beta-1 to mRNAs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q9BY77 |
| NCBI GenInfo Identifier: | 50403796 |
| NCBI Gene ID: | 84271 |
| NCBI Accession: | Q9BY77.2 |
| UniProt Secondary Accession: | Q9BY77,Q009A7, Q5H972, Q6PGN6, Q7Z6Z0, Q9NSP5, Q9NSP6 A8K6F8, A8K6V9, |
| UniProt Related Accession: | Q9BY77 |
| Molecular Weight: | 421 |
| NCBI Full Name: | Polymerase delta-interacting protein 3 |
| NCBI Synonym Full Names: | polymerase (DNA-directed), delta interacting protein 3 |
| NCBI Official Symbol: | POLDIP3Â Â |
| NCBI Official Synonym Symbols: | SKAR; PDIP46Â Â |
| NCBI Protein Information: | polymerase delta-interacting protein 3; RNA-binding protein P46; S6K1 Aly/REF-like target; 46 kDa DNA polymerase delta interaction protein |
| UniProt Protein Name: | Polymerase delta-interacting protein 3 |
| UniProt Synonym Protein Names: | 46 kDa DNA polymerase delta interaction protein; p46; S6K1 Aly/REF-like target; SKAR |
| Protein Family: | Polymerase delta-interacting protein |
| UniProt Gene Name: | POLDIP3Â Â |
| UniProt Entry Name: | PDIP3_HUMAN |
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