POLDIP3 Antibody (PACO55286)
- SKU:
- PACO55286
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- IP
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | POLDIP3 Antibody (PACO55286) |
| Antibody SKU: | PACO55286 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF, IP |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:20-1:200, IP:1:200-1:2000, |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Polymerase delta-interacting protein 3 protein (100-221AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate. All lanes: POLDIP3 antibody at 3.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 43 kDa. Observed band size: 43 kDa. |
 | Immunofluorescence staining of Hela cells with PACO55286 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunohistochemistry of paraffin-embedded human testis tissue using PACO55286 at dilution of 1:100. |
| Background: | Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Involved in nuclear mRNA export; probably mediated by assoociation with the TREX complex. |
| Synonyms: | Polymerase delta-interacting protein 3 (46 kDa DNA polymerase delta interaction protein) (p46) (S6K1 Aly/REF-like target) (SKAR), POLDIP3, KIAA1649 PDIP46 |
| UniProt Protein Function: | SKAR: Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Interacts with POLD2. Interacts with NCBP1 and EIF4A3. Associates with the multiprotein exon junction complex (EJC). Interacts with RPS6KB1 (activated). Interacts with ERH. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:RNA-binding Chromosomal Location of Human Ortholog: 22q13.2 Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nuclear speck Molecular Function:protein binding; nucleotide binding Biological Process: poly(A)+ mRNA export from nucleus; positive regulation of translation |
| NCBI Summary: | This gene encodes an RRM (RNA recognition motif)-containing protein that participates in the regulation of translation by recruiting ribosomal protein S6 kinase beta-1 to mRNAs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q9BY77 |
| NCBI GenInfo Identifier: | 50403796 |
| NCBI Gene ID: | 84271 |
| NCBI Accession: | Q9BY77.2 |
| UniProt Secondary Accession: | Q9BY77,Q009A7, Q5H972, Q6PGN6, Q7Z6Z0, Q9NSP5, Q9NSP6 A8K6F8, A8K6V9, |
| UniProt Related Accession: | Q9BY77 |
| Molecular Weight: | 421 |
| NCBI Full Name: | Polymerase delta-interacting protein 3 |
| NCBI Synonym Full Names: | polymerase (DNA-directed), delta interacting protein 3 |
| NCBI Official Symbol: | POLDIP3Â Â |
| NCBI Official Synonym Symbols: | SKAR; PDIP46Â Â |
| NCBI Protein Information: | polymerase delta-interacting protein 3; RNA-binding protein P46; S6K1 Aly/REF-like target; 46 kDa DNA polymerase delta interaction protein |
| UniProt Protein Name: | Polymerase delta-interacting protein 3 |
| UniProt Synonym Protein Names: | 46 kDa DNA polymerase delta interaction protein; p46; S6K1 Aly/REF-like target; SKAR |
| Protein Family: | Polymerase delta-interacting protein |
| UniProt Gene Name: | POLDIP3Â Â |
| UniProt Entry Name: | PDIP3_HUMAN |
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