The POLD2 Polyclonal Antibody (CAB6834) is a valuable tool for researchers studying POLD2, a protein involved in DNA replication and repair processes. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the POLD2 protein, this antibody allows for the detection and analysis of POLD2 expression in various cell types, making it an ideal choice for studies in molecular biology and cancer research.
POLD2 is a key component of the DNA polymerase delta complex, which is essential for accurately copying and repairing DNA. Dysregulation of POLD2 has been implicated in genomic instability and tumorigenesis, making it a potential target for cancer therapy research. By understanding the function and regulation of POLD2, researchers can gain valuable insights into mechanisms of DNA replication and potentially develop novel treatments for cancer and other diseases related to DNA damage.
Product Name:
POLD2 Rabbit Polyclonal Antibody
SKU:
CAB6834
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 120-360 of human POLD2 (NP_006221.2).
This gene encodes the 50-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA). Expression of this gene may be a marker for ovarian carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5.
Purification Method:
Affinity purification
Gene ID:
5425
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using POLD2 antibody (CAB6834) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
