POLA1 Antibody (PACO21724)
- SKU:
- PACO21724
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
POLA1 Antibody (PACO21724)
The Pola1 Antibody (PAC021724) is a critical tool for researchers studying Pola1, a key enzyme involved in DNA replication and repair processes. This polyclonal antibody, produced in rabbits, demonstrates high reactivity with Pola1 in human samples and is validated for use in Western blot applications. By specifically binding to the Pola1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for studies in molecular biology and cancer research.
Pola1 plays a crucial role in maintaining genomic stability and integrity by ensuring accurate DNA replication during cell division. Dysregulation of Pola1 has been implicated in various diseases, including cancer and genetic disorders, highlighting its importance as a potential therapeutic target. Investigating the function of Pola1 using this antibody is essential for gaining insights into the pathogenesis of these conditions and developing targeted treatments that exploit its role in DNA metabolism.
| Antibody Name: | POLA1 Antibody (PACO21724) |
| Antibody SKU: | PACO21724 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000 |
| Species Reactivity: | Human |
| Immunogen: | Synthesized peptide derived from N-terminal of human DNA Polymerase α . |
| Form: | Liquid |
| Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Hsi KL., Nucleic acid, Res. 18:6231-6237(1990). |
| Clonality: | Polyclonal |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from Jurkat cells, 293 cells and HepG2 cells, using DNA Polymerase alpha antibody. |
| Synonyms: | DNA pol alpha; DNA polymerase α catalytic subunit; DPOA; DPOLA; EC 2.7.7.7 |
| UniProt Protein Function: | POLA: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. Belongs to the DNA polymerase type-B family. |
| UniProt Protein Details: | Protein type:DNA replication; DNA repair, damage; Nuclear envelope; EC 2.7.7.7; Nucleotide Metabolism - purine; Transferase; Nucleotide Metabolism - pyrimidine Chromosomal Location of Human Ortholog: Xp22.1-p21.3 Cellular Component: nucleoplasm; alpha DNA polymerase:primase complex; nuclear matrix; cytoplasm; nucleolus; nuclear envelope; chromatin; nucleus Molecular Function:protein binding; DNA binding; DNA primase activity; protein heterodimerization activity; 4 iron, 4 sulfur cluster binding; metal ion binding; nucleoside binding; nucleotide binding; DNA-directed DNA polymerase activity; chromatin binding; protein kinase binding Biological Process: DNA replication initiation; viral reproduction; DNA replication, synthesis of RNA primer; DNA strand elongation during DNA replication; leading strand elongation; DNA repair; lagging strand elongation; double-strand break repair via nonhomologous end joining; telomere maintenance via semi-conservative replication; G1/S-specific transcription in mitotic cell cycle; DNA synthesis during DNA repair; cell proliferation; telomere maintenance via recombination; mitotic cell cycle; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle Disease: N Syndrome |
| NCBI Summary: | This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010] |
| UniProt Code: | P09884 |
| NCBI GenInfo Identifier: | 106507301 |
| NCBI Gene ID: | 5422 |
| NCBI Accession: | NP_058633 |
| UniProt Secondary Accession: | P09884,Q86UQ7, |
| UniProt Related Accession: | P09884 |
| Molecular Weight: | 165,913 Da |
| NCBI Full Name: | DNA polymerase alpha catalytic subunit isoform 2 |
| NCBI Synonym Full Names: | DNA polymerase alpha 1, catalytic subunit |
| NCBI Official Symbol: | POLA1Â Â |
| NCBI Official Synonym Symbols: | NSX; POLA; p180Â Â |
| NCBI Protein Information: | DNA polymerase alpha catalytic subunit |
| UniProt Protein Name: | DNA polymerase alpha catalytic subunit |
| UniProt Synonym Protein Names: | DNA polymerase alpha catalytic subunit p180 |
| Protein Family: | Polar flagellin |
| UniProt Gene Name: | POLA1Â Â |
| UniProt Entry Name: | DPOLA_HUMAN |
Related Products
POLA1 Antibody (PACO00714)
Ang4 Antibody (PACO64593)
SIM1 Antibody (PACO64949)
otsB Antibody (PACO64885)
