The POGlut1 Polyclonal Antibody (CAB17737) is a valuable tool for researchers studying POGlut1, an enzyme involved in post-translational modification of proteins through O-GlcNAcylation. This antibody is raised in rabbits and is highly reactive with human samples, making it suitable for a variety of research applications.POGlut1, also known as O-GlcNAc transferase, plays a crucial role in regulating cellular processes such as transcription, translation, and signaling pathways. Dysregulation of POGlut1 has been implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders. Therefore, investigating the function and expression of POGlut1 is important for understanding its role in disease pathogenesis.
The POGlut1 Polyclonal Antibody binds specifically to the POGlut1 protein, allowing for detection and analysis in different cell types and tissues. Its validation for use in techniques such as Western blotting makes it a reliable tool for studying the expression levels and localization of POGlut1 in various experimental settings. Researchers in fields such as cell biology, biochemistry, and molecular biology can benefit from using this antibody to further their understanding of POGlut1 and its implications in disease mechanisms.
Product Name:
POGLUT1 Rabbit Polyclonal Antibody
SKU:
CAB17737
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 160-280 of human POGLUT1 (NP_689518.1).
This gene encodes a protein with both O-glucosyltransferase and O-xylosyltransferase activity which localizes to the lumen of the endoplasmic reticulum. This protein has a carboxy-terminal KTEL motif which is predicted to function as an endoplasmic reticulum retention signal. This gene is an essential regulator of Notch signalling and likely plays a role in cell fate and tissue formation during development. It may also play a role in the pathogenesis of leukemia. Mutations in this gene have been associated with the autosomal dominant genodermatosis Dowling-Degos disease 4. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
56983
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using POGLUT1 Rabbit pAb (CAB17737) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
