The POFUT2 Polyclonal Antibody (CAB12223) is a valuable tool for researchers studying POFUT2, a key enzyme involved in protein glycosylation. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the POFUT2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for research in the fields of glycobiology and protein modification.POFUT2 is essential for the glycosylation of certain proteins, playing a crucial role in various cellular processes including cell signaling and adhesion.
Dysregulation of POFUT2 has been implicated in various diseases, making it a target of interest in studies of cancer, developmental disorders, and neurodegenerative diseases. Understanding the function of POFUT2 is essential for elucidating its role in disease pathogenesis and may ultimately lead to the development of novel therapeutic strategies targeting protein glycosylation pathways.
Product Name:
POFUT2 Rabbit Polyclonal Antibody
SKU:
CAB12223
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 240-370 of human POFUT2 (NP_056042.1).
Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).
Purification Method:
Affinity purification
Gene ID:
23275
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using POFUT2 Rabbit pAb (CAB12223) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
