The Podoplanin Polyclonal Antibody (CAB21748) is a valuable tool for researchers studying podoplanin, a glycoprotein involved in cell adhesion and platelet aggregation. This antibody, generated in rabbits, exhibits high reactivity with human samples and is optimized for Western blot applications. By binding to the podoplanin protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in cancer biology and vascular biology.Podoplanin is known for its role in promoting tumor progression and metastasis, as well as its involvement in lymphatic vessel formation and maintenance.
Its expression is commonly upregulated in various types of cancer, making it a promising target for therapeutic intervention. Studying the function of podoplanin using this antibody can provide valuable insights into the mechanisms underlying cancer progression and metastasis, offering potential opportunities for the development of novel cancer treatments.
Product Name:
Podoplanin Polyclonal Antibody
SKU:
CAB21748
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 99-199 of human Podoplanin (NP_006465.3).
Sequence:
ASTG QPED DTET TGLE GGVA MPGA EDDV VTPG TSED RYKS GLTT LVAT SVNS VTGI RIED LPTS ESTV HAQE QSPS ATAS NVAT SHST EKVD GDTQ TTVE K
Cell projection, Membrane, Single-pass type I membrane protein, filopodium membrane, lamellipodium membrane, microvillus membrane, ruffle membrane.
Calculated MW:
17kDa
Observed MW:
36kDa
This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
10630
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from U2OS cells, using Podoplanin Rabbit pAb (CAB21748) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
