Thank you for providing the URL for the POC1B Polyclonal Antibody (CAB7616) from AssayGenie. This antibody is designed for research involving POC1B, a protein associated with centriole biogenesis and ciliogenesis. The antibody, raised in rabbits, is highly specific to human samples and is suitable for use in Western blot applications. By binding to the POC1B protein, this antibody enables accurate detection and analysis of POC1B expression in various cell types, making it an essential tool for studies in cell biology and developmental biology.POC1B is a key player in centriole and ciliary functions, playing a critical role in the assembly and maintenance of these cellular structures. Dysfunction of POC1B has been linked to various ciliopathies, developmental disorders, and cancers, making it a promising target for research in these areas.
By understanding the functions and regulation of POC1B, researchers can gain valuable insights into cellular processes and potentially develop new strategies for treating related diseases.In summary, the POC1B Polyclonal Antibody (CAB7616) offers researchers a reliable tool for studying POC1B expression and function in various biological contexts. Its high specificity and sensitivity make it a valuable asset for investigations into centriole biogenesis, ciliogenesis, and related pathways, paving the way for advancements in cell biology and disease research.
Product Name:
POC1B Rabbit Polyclonal Antibody
SKU:
CAB7616
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 299-478 of human POC1B (NP_758440.1).
Cytoplasm, centriole, centrosome, cilium basal body, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
54kDa
Observed MW:
54kDa
POC1 proteins contain an N-terminal WD40 domain and a C-terminal coiled coil domain and are part of centrosomes. They play an important role in basal body and cilia formation. This gene encodes one of the two POC1 proteins found in humans. Mutation in this gene result in autosomal-recessive cone-rod dystrophy. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
282809
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using POC1B antibody (CAB7616) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
