The PNPO Polyclonal Antibody (CAB18495) is a vital tool for researchers studying pyridoxine-5'-phosphate oxidase (PNPO), an essential enzyme involved in vitamin B6 metabolism. This antibody, developed in rabbits, offers high reactivity with human samples and is validated for use in Western blotting applications. By specifically binding to the PNPO protein, this antibody enables accurate detection and analysis in various cell types, making it an invaluable asset for studies in biochemistry and metabolism research.PNPO plays a crucial role in the conversion of pyridoxine to pyridoxal 5'-phosphate, the biologically active form of vitamin B6. Dysregulation of PNPO activity has been linked to various neurological disorders, making it a promising target for therapeutic interventions.
Understanding the function and regulation of PNPO is essential for developing treatments for conditions such as epilepsy, autism, and other vitamin B6-related disorders.With its high specificity and sensitivity, the PNPO Polyclonal Antibody (CAB18495) is a reliable tool for researchers aiming to unravel the roles and mechanisms of PNPO in health and disease. Its versatility and performance make it a valuable asset for laboratories conducting cutting-edge research in biochemistry and molecular biology.
Product Name:
PNPO Rabbit Polyclonal Antibody
SKU:
CAB18495
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PNPO (NP_060599.1).
The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy.
Purification Method:
Affinity purification
Gene ID:
55163
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PNPO Rabbit pAb (CAB18495) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
